Microvascular Research Laboratories

Publications (Sep 2006)

A.V. Benest, S.J.Harper, S.Yla Herttuala, K. Alitalo, D.O. Bates Angiogenesis following VEGF-C overexpression is contextually dependent on lymphangiogenesis. Cardiovascular Research in press.

Y. Qiu, H.S. Bevan, S. Weeraperuma, D. Wratting, D. Murphy, C.R. Neal, D.O. Bates, S J. Harper, Mammary alveolar development during lactation is inhibited by the endogenous anti-angiogenic growth factor isoform, VEGF₁₆₅b. Faseb Journal. In press.

H S. Bevan, S J. Harper, D O. Bates Vascular Endothelial Growth Factors ’Äì Molecular diversity and function. In: Angiogenesis: Basic Science and Clinical Applications, 2007 Editors: M. E. Maragoudakis and E. Papadimitriou. ISBN: 978-81-7895-302-1 Pub. Transworld Research Network

AHJ Salmon, I Toma, R Muston, SJ Harper, DO Bates, CR Neal, J Peti-Peterdi. Evidence for restriction of fluid and solute movement across the glomerular capillary wall by the sub podocyte space: a new layer in the glomerular filtration barrier. Am J Physiol 2007. 293: F1777-86

C.R. Neal, R. Muston, D. Njegovan, R. Ferrell, S.J Harper, W.M. Deen, D. O Bates. Glomerular filtration into the sub-podocyte space is highly restricted under physiological perfusion conditions. Am J Physiol 2007. 293: F1787-98.

AV Benest, DO Bates Measurement of Angiogenic phenotype by use of two dimensional mesenteric angiogenesis assay. In: Angiogenesis Protocols, Ed Martin. in Press

R M. Perrin, S J. Harper and D O. Bates Microvascular permeability and diabetes mellitus. Cell Biochem Biophys. 2007. 49(2):65-72.

RO Pritchard-Jones, D.B.A. Dunn, Y Qiu, AHR Varey, Orlando, H Rigby, S J Harper, D O Bates, Expression of VEGF_xxxb, the inhibitory isoforms of VEGF, in malignant melanoma. Br J Cancer. 2007. 97(2):223-30.

SE Cross, SK Richards, A Clark, AV Benest, DO Bates, PW Mathieson, PRV Johnson, SJ Harper and RM Smith. Vascular endothelial growth factor as a survival factor for human islets; effect of immunosuppressive drugs. Diabetologia, 2007. 50(7):1423-32

R M Perrin, R Corrall, D O Bates, S J Harper. Hyperglycaemia increases microvascular exchange by increasing convective water permeability. Microcirculation 2007. 14(7):683-96..

J D. Shields, M. Emmett, DBA Dunn, K D. Joory, H Rigby, P S. Mortimer, A Orlando, J. R Levick, D O. Bates. Migration of melanoma cells towards lymphatics Ôø‡Ôø‡Ôø‡ a mechanism underlying metastasis. Oncogene. 2007: 26(21) 2997-3005.

S J Harper, D O Bates Vascular Endothelial Growth Factor splicing and its potential role in cancer progression. In: Splicing in Cancer. Ed J Venables.

M. Ladomery, S.J. Harper, D.O. Bates. Alternative splicing in angiogenesis: the VEGF paradigm. Cancer Letters. (2007) 249: 133-42

H.W. Cheng, A.F. James, R.R. Foster, J.C. Hancox, D.O. Bates. VEGF activates receptor-operated cation channels in human microvascular endothelial cells. Arterioscler Thromb Vasc Biol. 2006 26(8):1768-76

O. Konopatskaya, S.J. Harper, A.J. Churchill D.O. Bates, T.A. Gardiner. VEGF165b, an endogenous C-terminal splice variant of VEGF, inhibits retinal neovascularisation in mice. Molecular Vision. 2006; 12:626-632

A.J. Hill, J.M. Hinton, H-W Cheng, D.O. Bates, P.D. Langton, A.F. James. Function and expression of TRPC channels in rat mesenteric arteries: Contribution to a vascular myocyte a1-adrenoceptor-operated current. Cell Calcium. 2006 40(1):29-40

A.V. Benest, A.H.J. Salmon, W-Y. Wang C.P. Glover, J. Uney, S.J. Harper, D.O. Bates. Vascular Endothelial Growth Factor and Angiopoietin-1 stimulate different angiogenic phenotypes that synergise to form a functional vasculature. Microcirculation. 2006 Sep;13(6):423-37

R.R. Foster, M.A. Saleem, P.W. Mathieson D.O. Bates and S.J. Harper. VEGF-C promotes survival in podocytes. Am J Physiol: Renal Physiol.

C.A. Glass, S.J. Harper, D.O. Bates. The anti-angiogenic vascular endothelial growth factor isoform, VEGF165b transiently increases hydraulic conductivity, probably through VEGF receptor 1 in vivo. J Physiol. 2006

D.O. Bates, P.P MacMillan, J.G. Manjaly, Y. Qiu, S.J. Hudson, A.J. Hunter, P. Soothill, C. Reid, L.F. Donaldson, S.J. Harper. The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down regulated in pre-eclamptic placentae at term. Clinical Science. 2006 110(5): 575-585

U. Fiedler, S. Christian, S. Koidl, D. Kerjaschki, D.O. Bates, G. Christofori, and H. G. Augustin. The sialomucin CD34 is a marker of tumor-associated lymphatic endothelial cells in human tumors. Am J Pathol. 2006 168(3):1045-53.

A.H.J. Salmon, C.R. Neal, D.O Bates, S.J. Harper Vascular endothelial growth factor (VEGF165) increases the ultrafiltration coefficient in isolated intact rat glomeruli. J Physiol. 2006. 570:141-56

C.A. Glass, T.M. Pocock, D. O. Bates Transient osmotic absorption of fluid in microvessels exposed to low concentrations of dimethylsulphoxide (DMSO). Microcirculation. 2006. 13(1):29-40.

2005

D.O. Bates. Regulation of Microvascular Permeability. In: Encyclopedia of the Microvasculature. Ed. D.N. Granger, D. Shepro. Elsevier.

D.O Bates, S.J. Harper. Therapeutic potential of inhibitory VEGF splice variants. Future Oncology. 2005. 1 (4), 467-473

R.M. Perrin, O. Konopatskaya, Y. Qiu, S. Harper, D.O. Bates, A.J. Churchill Diabetic retinopathy is associated with a switch in splicing from anti- to pro- angiogenic isoforms of vascular endothelial growth factor. Diabetologia. 48(11):2422-7

C.A. Glass, T.M. Pocock, F.E. Curry, D.O. Bates. Cytosolic Ca2+ concentration and the rate of increase of the cytosolic Ca2+ concentration in the regulation of vascular permeability in vivo. J Physiol. 2005. 564.3: 817-827.

C R Neal, H Crook, E Bell, S J Harper, D O Bates. 3D reconstruction of glomeruli by electron microscopy reveals a distinct restrictive urinary sub-podocyte space. J Am Soc Nephrol, 2005. 16(5):1223-35

R.J.M. Coward, R.R Foster, D. Patton, F.W.L. Ni, D.O. Bates, S.J. Harper, P.W. Mathieson, M.A. Saleem. Nephrotic plasma alters slit diaphragm dependent signalling, and localisation of nephrin, podocin and CD2AP in the human podocyte. J Am Soc Nephrol. 2005 16(3):629-37

R.R. Foster, M.A. Saleem, P.W. Mathieson, D.O. Bates, S.J. Harper Vascular endothelial growth factor acts through nephrin to reduce apoptosis in human podocytes. American Journal of Physiology: Renal Physiology 2005. 288: F48-57.

2004

J Woolard, W-Y Wang, Y Qiu, HS Bevan, L Morbidelli, RO Pritchard Jones, JD Shields, T-G Cui, M Sugiono, E Waine, R Perrin, RR Foster, J Digby-Bell, C E Whittles, R Mushens, D A Gillatt, M Ziche, S J Harper, D O Bates. Inhibitory VEGF splice variants. VEGF165b, an inhibitory vascular endothelial growth factor splice variant: mechanism of action, in vivo effect on angiogenesis and endogenous protein expression. Cancer Research. 64(21):7822-35

Barker AL, Konopatskaya O, Neal CR, Macpherson JV, Whatmore JL, Winlove CP, Unwin PR, Shore AC. Observation and characterisation of the glycocalyx of viable human endothelial cells using confocal laser scanning microscopy. Physical Chemistry Chemical Physics. 6 (5): 1006-1011 2004

C.R. Neal, A.J. Hunter, S J Harper, P W Soothill, D O Bates. Plasma from women with severe pre-eclampsia increases microvascular permeability in an animal model in vivo. Clinical Science. 2004: 107(4) 399-405.

C.A. Glass, D.O. Bates. The role of release of endothelial cell Ca²⁺ store release in the regulation of microvascular permeability in vivo. Experimental Physiology 2004: 89(4):343-51.

W. Wang, C.E. Whittles, S.J. Harper, D.O. Bates. An adenovirus mediated gene transfer model of angiogenesis in rat mesentery. Microcirculation. 2004: 11 (4) 361-375,

T-G Cui, RR Foster, M Saleem, PW Mathieson, DA Gillatt, DO Bates, SJ Harper. Differentiated human podocytes endogenously express an inhibitory isoform of vascular endothelial growth factor (VEGF165b) mRNA and protein. American Journal of Physiology: Renal Physiology 2004 Apr;286(4):F767-73

T.M.Pocock, R.R. Foster, D.O. Bates Evidence of a role for TRPC channels in vascular endothelial growth factor mediated increased vascular permeability in vivo. American Journal of Physiology, Heart and Circ Physiol. (2004) 286: Mar H1015Ôø‡Ôø‡Ôø‡H1026

J.D. Shields, M Borsetti, H Rigby, SJ Harper, PS Mortimer, J.R Levick, A Orlando, DO. Bates. Lymphatic density and metastatic spread in human malignant melanoma. Brit. J. Cancer, (2004) 90: Feb. 693-700

A.A. Okeke, D.O. Bates, D.A. Gillatt. Lymphoedema in urological cancer. European Urology. 2004 Jan; 45(1): 18-25

2003

D.O. Bates. ZD-6474, an inhibitor of VEGF-R2, as an anti-angiogenic agent. Current Opinions in Investigational Drugs. 2003 4 (12).

D O Bates, R O Pritchard-Jones. The role of VEGF in wound healing. International Journal of Lower Extremity Wounds. 2003; 2(2): 107-120

D.O. Bates S.J. Harper, Regulation of microvascular permeability by vascular endothelial growth factors. Vascular Pharmacology. (2003) 39(4-5) 225-23

Harper SJ, Bates DO. Endothelial permeability in uremia. Kidney International Supplement. 2003 May;(84):41-4.

Bates DO. Pre-eclampsia and the microcirculation: a novel explanation. Clinical Science (Lond). 2003 Apr;104(4):413-4.

Foster RR, Hole R, Anderson K, Satchell SC, Coward RJ, Mathieson PW, Gillatt DA, Saleem MA, Bates DO, Harper SJ.Functional evidence that vascular endothelial growth factor may act as an autocrine factor on human podocytes. American Journal of Physiology Renal Physiology. 2003 Jun;284(6):F1263-73.

Glass CA, Bates DO. Role of endothelial Ca2+ stores in the regulation of hydraulic conductivity of Rana microvessels in vivo. American Journal of Physiology Heart Circulatory Physiology 2003 Apr;284(4):H1468-78

2002

Bates DO, Harper SJ. Regulation of vascular permeability by vascular endothelial growth factors. Vascular Pharmacology. 2002 Nov;39(4-5):225-37.

Whittles CE, Pocock TM, Wedge SR, Kendrew J, Hennequin LF, Harper SJ, Bates DO. ZM323881, a novel inhibitor of vascular endothelial growth factor-receptor-2 tyrosine kinase activity. Microcirculation. 2002 Dec;9(6):513-22.

C.R. Neal, D.O. Bates Measurement of hydraulic conductivity of single perfused mesenteric microvessels between periods of controlled shear stress. Journal of Physiology (2002), 543 (3), 947-957.

D.O. Bates, T-G. Cui, J.M. Doughty, M. Winkler, M. Sugiono, J.D. Shields, D. Peat, D.A. Gillatt, S.J Harper. VEGF165b - an inhibitory splice variant of vascular endothelial growth factor is downregulated in renal cell carcinoma. Cancer Research. (2002) 62(14):4123-31.

D. O. Bates, N. J. Hillman, B. Williams, C. R. Neal and T. M. Pocock. Regulation of microvascular permeability by vascular endothelial growth factors Journal of Anatomy. (2002) 200(6) 581-598.

Harper SJ, Tomson CV, D.O. Bates. Human uraemic plasma increases microvascular permeability to water and proteins in vivo. Kidney International (2002): 61(4): 1416-22

2001

D.O. Bates. Novel insight into the pathophysiology of breast cancer related lymphoedema. Clinical Science (2001): 101(2) 169-170

T.M. Pocock, D.O. Bates. In vivo mechanisms of vascular endothelial growth factor mediated increased hydraulic conductivity of Ranacapillaries. Journal of Physiology (2001) 534(2) 479-488.
N.J. Hillman, C.E. Whittles, T.M. Pocock, B Williams, D.O. Bates Differential effects of vascular endothelial growth factor-C and placental growth factor-1 on the hydraulic conductivity of frog mesenteric capillaries.. J. Vasc. Res. (2001) 38: 176-186

D.O. Bates, R.I. Heald, B. Williams, F.E. Curry. The mechanism of Vascular Endothelial Growth Factor induced chronically increased vascular permeability and compliance. Journal of Physiology (2001) 533(1) 263-272.

1996-2000

T.M. Pocock, B. Williams, F.E. Curry, D.O. Bates. VEGF and ATP act by different mechanisms to increase microvascular permeability and endothelial [Ca2+]i American Journal of Physiology (2000) 279(4):H1625-H1634

D.O. Bates, D Lodwick, B Williams. Vascular Endothelial Growth Factor And Microvascular Permeability. Microcirculation. (1999) 6: 83-96

D.O. Bates. The chronic effect of vascular endothelial growth factor (VEGF) on individually perfused frog mesenteric microvessels. Journal of Physiology : (1998) 513:
225-233

D.O. Bates, F.E. Curry. Vascular endothelial growth factor increases microvascular permeability via a calcium dependent pathway. American Journal of Physiology. (1997) 273: H687-694

D.O. Bates, F.E. Curry. Vascular endothelial growth factor increases hydraulic conductance of isolated perfused microvessels. American Journal of Physiology. (1996) 271: H2520-2528

P.S. Mortimer, D.O. Bates, H. Brassington, AWB Stanton, D. Strachan, J.R. Levick., The Prevalence of Arm Oedema Following Treatment for Breast Cancer. Quart Journal of Medicine. (1996) 89: 377-380.

1992-1995

D.O. Bates, A.W.B. Stanton, J.R. Levick, P.S. Mortimer. The Effect Of Hosiery On Interstitial Fluid Pressure And Diurnal Arm Volume Fluctuations In Arm Lymphoedema. Phlebology (1995): 10: 46-50

D.O. Bates J.R. Levick, P.S. Mortimer. Starling pressures in the human arm and their alteration in postmastectomy oedema. Journal of Physiology (1994) 477 (2):355-363.

D.O. Bates J.R. Levick, P.S. Mortimer. Quantification of rate and depth of pitting in human oedema using an electronic tonometer. Lymphology (1994) 27(4):159-72

J.R. Levick , P.S. Mortimer, D.O. Bates. Spatial Filtration-Reabsorption Balance Along The Capillary Axis - Fact Or Fiction. Lymphology, (1994), 27S : 460-463.

D.O. Bates, J.R. Levick P.S. Mortimer. Biochemical macromolecular composition of interstitial fluid from women following breast cancer treatment and implications for microvascular exchange. Clinical Science (1993) 85(6):737-46

D.O. Bates J.R. Levick P.S. Mortimer. Colloid osmotic pressure (COP), protein concentration and interstitial fluid pressure in lymphoedema. Progress in Lymphology (1993) 994:167-170.

D.O. Bates J.R. Levick P.S. Mortimer. An instrument for determination of pitting characteristics in lymphoedema. Progress in Lymphology (1993) 994: 579-80.

O. Luetolf, R.H. Bull, D.O. Bates, P.S. Mortimer. Capillary under perfusion in chronic venous insufficiency: a cause for leg ulceration? Br J Dermatology (1993) 128 249-254

R.H.Bull, D.O. Bates, P.S. Mortimer. Intravital video capillaroscopy for the study of the microcirculation in psoriasis. Br J Dermatology (1992) 126: 436-445.

D.O. Bates J.R. Levick P.S. Mortimer. Subcutaneous interstitial fluid pressure and arm volume in lymphoedema. Int. J Microcirc.: Clin. & Exp. (1992) 11: 287-295.

D.O. Bates. Starling Forces in Lymphoedema. Ph.D. Thesis London 1992.

Recent Abstracts (1995-)

Pocock T.M., D.O. Bates. U73122, an inhibitor of phospholipase C (PLC) inhibits VEGF mediated increases in hydraulic conductivity. Faseb J (2000) 14: A21

Bates, D.O. Chronically increased microvascular hydraulic conductivity but not diameter or distensibility, are attenuated by acute ca2+ influx inhibition. J. Vasc Res. (2000) in press
.
Hillman, N.J. T.L. de Haro, B. Williams and D.O. Bates, Microvessel endothelial cell ultrastructure after vascular endothelial growth factor-c (VEGF-C) induced chronically increased Lp J. Vasc Res. (2000) in press

Pocock T.M., B. Williams, D.O. Bates. Increased microvascular hydraulic conductivity (Lp) induced by ATP is attenuated by inhibition of phospholipase C (PLC). J. Vasc Res. (2000) in press

Bates, D.O. Chronically increased hydraulic conductivity (Lp) by vascular endothelial growth factor (VEGF) is attenuated by inhibition of calcium influx.J Physiol . (2000) in press

Hillman, N.J., R.I. Heald, B. Williams, D.O Bates. Microvascular hydraulic conductivity (Lp) is chronically increased 24 hours after perfusion with vascular endothelial growth factor C (VEGF-C) but not after perfusion with Placental Growth Factor (PlGF)J Physiol . (2000) in press

T.M. Pocock, F.E. Curry*, +B. Williams, D.O. Bates. VEGF increases endothelial intracellular cell calcium concentration ([Ca2+]i) in frog mesenteric microvessels in vivo by a mechanism which is calcium store independent.J Physiol . (2000) in press

Bates, D. O. The effect of sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) inhibition on vascular endothelial growth factor (VEGF) mediated increased microvascular hydraulic conductivity (Lp). J Physiol 518: 82, 1999.

Hillman, N., B. Williams, and D. Bates. Differential effects of vascular endothelial growth factor-C (VEGF-C) and placental growth factor (PlGF) on hydraulic conductivity (Lp) of frog mesenteric microvessels. J Physiol 518: 83, 1999.

Pocock, T., F. Curry, B. Williams, and D. Bates. The effect of sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) inhibition on the endothelial cell calcium concentration ([Ca2+]i) of frog mesenteric microvessels. J Physiol 518: 82, 1999.

N.J. Hillman, M. Tas*, R.C. Murray*, B. Williams and D.O. Bates. Endothelial Monocyte Activating Polypeptide-2 (EMAP-2) Increases Hydraulic Conductivity (Lp) of Isolated Perfused Frog Mesenteric Microvessels after Perfusion with Whole Blood. Journal of Physiology. (1998) 513P: 154-5P

Bates, D. O. Chronically increased hydraulic conductivity (Lp) by vascular endothelial growth factor (VEGF) is attenuated by inhibition of nitric oxide synthase Journal of Vascular Research (1998).35: (5) 369.

Bates, D. O.Vascular endothelial growth factor (VEGF) chronically increases microvascular hydraulic conductivity (Lp) without affecting oncotic reflection coefficient (s). Faseb Journal . (1998)12: A22.

Bates, D.O. and F.E. Curry. Microvascular hydraulic conductivity (Lp) is chronically increased by acute, transient perfusion with adenosine triphosphate (ATP). Int. J. Microcirc. 1997 17 (4) 269

Bates, D.O. and F.E. Curry. Vascular Endothelial Growth Factor (VEGF) Increases Microvascular Hydraulic Conductance (Lp) Via Increased Calcium Influx. Microcirculation 1997 4(1) 147

Bates, D.O. and F.E. Curry. Vascular endothelial growth factor (VEGF) induced increased hydraulic conductivity (Lp) does not display tachyphylaxis after ten minutes. Int. J. Microcirc (1996) 16(S1) 92

Bates, D.O. and F.E. Curry. Vascular endothelial growth factor acutely and chronically increases hydraulic conductance (Lp) of perfused microvessels. Microcirculation, (1996) 3(1) 80

Bates, D.O. and F.E. Curry, Exogenous gene expression in mesenteric microvessels, and its effect on transvascular fluid transport. Microcirculation, 1995. 2(1): p. 71.

Departmental Home page Dave's Home Page

Last updated: 28June199916:50

Dr D.O. Bates